Maverick Vinales je s časom 1:32.638 dobil drugi trening v Assenu ter ob tem le za 0,011 sekunde zaostal za rekordom steze Valentina Rossija. Na drugem mestu se mu je najbolj uspel približati Fabio Quartararo, ki je za njim zaostal 0,180 sekunde. Tretje in četro mesto sta zasedla tovarniška dirkača Ducatija, Petrucci pred Doviziosom. Alex Rins je tokrat postavil peti čas ter tako zaključil tik pred nekoliko presenetljivo Andreo Iannonejem, ki je sicer padel s devetem zavoju. Aktualni svetovni prvak Marc Marquez je tokrat postavil sedmi čas.
Deseterico so zaključili Joan Mir, Valentino Rossi ter Cal Crutchlow.
| Poz | Dirkač | Drž | Moštvo | Čas | Krog | ||
| 1 | ^1 | Maverick Viñales | SPA | Monster Yamaha (YZR-M1) | 1’32.638s | 21/23 | |
| 2 | ˅1 | Fabio Quartararo | FRA | Petronas Yamaha (YZR-M1)* | 1’32.818s | 19/20 | |
| 3 | = | Danilo Petrucci | ITA | Mission Winnow Ducati (GP19) | 1’32.952s | 20/20 | |
| 4 | ^9 | Andrea Dovizioso | ITA | Mission Winnow Ducati (GP19) | 1’33.223s | 6/18 | |
| 5 | ˅1 | Alex Rins | SPA | Suzuki Ecstar (GSX-RR) | 1’33.305s | 21/21 | |
| 6 | ^5 | Andrea Iannone | ITA | Factory Aprilia Gresini (RS-GP) | 1’33.355s | 6/15 | |
| 7 | ˅1 | Marc Marquez | SPA | Repsol Honda (RC213V) | 1’33.413s | 6/21 | |
| 8 | ^8 | Joan Mir | SPA | Suzuki Ecstar (GSX-RR)* | 1’33.415s | 19/21 | |
| 9 | ^3 | Valentino Rossi | ITA | Monster Yamaha (YZR-M1) | 1’33.517s | 23/23 | |
| 10 | ˅3 | Cal Crutchlow | GBR | LCR Honda (RC213V) | 1’33.727s | 20/20 | |
| 11 | ^10 | Francesco Bagnaia | ITA | Pramac Ducati (GP18)* | 1’33.815s | 17/18 | |
| 12 | ˅4 | Karel Abraham | CZE | Reale Avintia Ducati (GP18) | 1’33.854s | 17/17 | |
| 13 | ˅3 | Jack Miller | AUS | Pramac Ducati (GP19) | 1’33.874s | 18/20 | |
| 14 | ^3 | Franco Morbidelli | ITA | Petronas Yamaha (YZR-M1) | 1’33.963s | 7/23 | |
| 15 | ˅6 | Pol Espargaro | SPA | Red Bull KTM Factory (RC16) | 1’33.971s | 18/18 | |
| 16 | ˅11 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | 1’34.040s | 21/21 | |
| 17 | ^2 | Johann Zarco | FRA | Red Bull KTM Factory (RC16) | 1’34.194s | 14/14 | |
| 18 | ˅3 | Tito Rabat | SPA | Reale Avintia Ducati (GP18) | 1’34.370s | 22/22 | |
| 19 | ˅5 | Aleix Espargaro | SPA | Factory Aprilia Gresini (RS-GP) | 1’34.537s | 16/16 | |
| 20 | = | Miguel Oliveira | POR | Red Bull KTM Tech3 (RC16)* | 1’34.628s | 19/19 | |
| 21 | ^1 | Hafizh Syahrin | MAL | Red Bull KTM Tech3 (RC16) | 1’35.250s | 5/17 | |
| 22 | ˅4 | Jorge Lorenzo | SPA | Repsol Honda (RC213V) | No Time | 0/0 |
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